Comparison of PAM50 classification and proteome profiles in breast cancer tissue

Introduction

PAM50 classification

Based on microarray or RNA-seq data, looking at 50 representative genes to classify breast-cancer tissues, classify samples into four categories:

  • HER2-enriched
  • Luminal A
  • Luminal B
  • Basal like

Our data

iTRAQ-based mass spectrometry proteome profiling data from 77 breast cancer tissues1

Tumor receptor status for:

  • Oestrogen (ER)
  • Progesterone (PR)
  • Human epidermal growth factor receptor 2 (HER2)

Methods and strategy

Some text about our approach, or a flow chart

Results

Clustering based on entire data set

Differential gene expression analysis

Differential gene expression analysis using linear models: \(lm(PAM50 \sim protein)\)

Results from differential gene expression analysis

PCA with significant proteins

Hierachial clustering with significant proteins

Clinical data correlation

Proteomics data correlation

  • What did we do
  • What did we see
  • This is nice
  • we can also leave out the bullet list

Conclusion & Discussion

  • As expected, no clustering was seen when analysing the full proteomics data set.
  • Considering only the differentially expressed genes, we could some-what replicate the PAM50 classification.
  • In addition, we investigated correlations […]

Group 17, R for Bio Data Science